During an SPR experiment, there are many experimental parameters that must be controlled in order to optimize the performance of the system and to generate high-quality binding data. Of these parameters, minimizing non-specific binding (NSB) is one of the most imperative as it can directly affect the accuracy of your kinetic data. SPR experiments typically…
SPR is a label-free technique that enables you to get in depth information on how two biomolecules are interacting. This is done on a sensor inside of an instrument such as the OpenSPR or Alto. SPR instruments are primarily used to measure binding affinity and kinetics, and are becoming increasingly more affordable and accessible as…
Read on to learn about the two most common approaches for measuring binding kinetics, multi-cycle kinetics (MCK) and single-cycle kinetics (SCK), and how to select the right one for your interaction.
Measure Affinity and Kinetics in Real Time from Your Bench SPR assays are a gold standard in biologics research and development of safe and effective treatments against disease. The data derived from SPR can aid us in better understanding molecular mechanisms while providing inishgt into key signaling pathways and advancing drug development. Join us for this…
Understanding the signaling pathways that the biomarkers can activate can help in the development of check-point immunotherapies. Surface Plasmon Resonance (SPR), is a common technique used to identify and elucidate biomarker interactions, signaling pathways, mechanism of interaction, drug discovery and development of therapeutics by providing a label-free, real-time method of determining binding kinetics and binding…
Downloads Detection of Pressure-Induced Aggregation Using Microfluidic Modulation Spectroscopy (MMS) (PDF, 1 MB) Pressure-Induced Protein Aggregation Detection: Protein aggregation is a recognized signal of instability and can lead to the loss of protein function. It is therefore crucial to detect protein aggregation early in the drug development process to inform further drug development decisions. Pressure,…
Downloads Delineating the conformational landscape of the adenosine A2A receptor during G protein coupling (PDF, 9 MB) G-protein-coupled receptors (GPCRs) represent a ubiquitous membrane protein family and are important drug targets. Their diverse signaling pathways are driven by complex pharmacology arising from a conformational ensemble rarely captured by structural methods. Here, fluorine nuclear magnetic resonance spectroscopy (19F NMR) is used to delineate key…
Downloads Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS) (PDF, 1 MB) Biosimilar molecules are designed to mimic innovator molecules that have been approved and are available on the market, providing others within the industry an opportunity to create structurally similar therapeutic medicines for greater patient…
Existing tools for measuring and monitoring the structure of biotherapeutic molecules during formulation have had until now very significant limitations in sensitivity and have been very difficult to use. In this webinar, Amgen shares data that demonstrates the power of Microfluidic Modulation Spectroscopy (MMS), a novel, fully-automated IR technology, to generate high-quality, reproducible secondary structure…
Gene therapy involves introducing new genetic material into cells so that abnormal genes causing bad protein-coding functions are altered, replaced or silenced. It is most active in the treatment of cancer, accounting for over 60 percent of clinical trials and over 50 percent of all approved therapies worldwide. Starting in the early 2000s, countries in…