Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis
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Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis

Bylejin.varghese

Quick, label-free methods with minimal sample prep for simultaneously measuring rAAV aggregation, capsid charge, and particle concentration. Date recorded: July 15, 2021 Viral vectors, such as Recombinant Adeno-Associated Viruses (rAAV) Adenoviruses (AV) are increasingly being used in the development of gene therapies and vaccines. The efficient development of viral vectors order to develop effective therapies and…

Master Lipid Nanoparticle & Liposome Characterization with Light Scattering
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Master Lipid Nanoparticle & Liposome Characterization with Light Scattering

Bylejin.varghese

Recent breakthroughs with mRNA-based vaccines highlight the potential of lipid-based particles as powerful and versatile delivery vectors for vaccines and gene therapies. Used to measure particle size, stability, zeta potential and particle concentration, light scattering techniques are used extensively in the characterization of lipid nanoparticle and liposome research. The Zetasizer range of light scattering instruments…

4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments
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4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments

Bylejin.varghese

During an SPR experiment, there are many experimental parameters that must be controlled in order to optimize the performance of the system and to generate high-quality binding data. Of these parameters, minimizing non-specific binding (NSB) is one of the most imperative as it can directly affect the accuracy of your kinetic data. SPR experiments typically…

Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists
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Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists

Bylejin.varghese

SPR is a label-free technique that enables you to get in depth information on how two biomolecules are interacting. This is done on a sensor inside of an instrument such as the OpenSPR or Alto. SPR instruments are primarily used to measure binding affinity and kinetics, and are becoming increasingly more affordable and accessible as…

Analyzing Binding Kinetics: Which Method Is Best for Your Next SPR Experiment?
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Analyzing Binding Kinetics: Which Method Is Best for Your Next SPR Experiment?

Bylejin.varghese

Read on to learn about the two most common approaches for measuring binding kinetics, multi-cycle kinetics (MCK) and single-cycle kinetics (SCK), and how to select the right one for your interaction.