Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis
| | |

Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis

Bylejin.varghese

Quick, label-free methods with minimal sample prep for simultaneously measuring rAAV aggregation, capsid charge, and particle concentration. Date recorded: July 15, 2021 Viral vectors, such as Recombinant Adeno-Associated Viruses (rAAV) Adenoviruses (AV) are increasingly being used in the development of gene therapies and vaccines. The efficient development of viral vectors order to develop effective therapies and…

Master Lipid Nanoparticle & Liposome Characterization with Light Scattering
| | |

Master Lipid Nanoparticle & Liposome Characterization with Light Scattering

Bylejin.varghese

Recent breakthroughs with mRNA-based vaccines highlight the potential of lipid-based particles as powerful and versatile delivery vectors for vaccines and gene therapies. Used to measure particle size, stability, zeta potential and particle concentration, light scattering techniques are used extensively in the characterization of lipid nanoparticle and liposome research. The Zetasizer range of light scattering instruments…

Bispecific Antibodies and Antibody Drug Conjugates: More Complex and Targeted Biologic Therapeutics
| | | | |

Bispecific Antibodies and Antibody Drug Conjugates: More Complex and Targeted Biologic Therapeutics

Bylejin.varghese

With the maturation of monoclonal antibodies (mAbs) based cancer immunotherapy drugs that target specific, actionable biomarkers, the biopharmaceutical industry has been developing more complex mAbs, such as bispecific antibodies and antibody drug conjugates. The Business Research Company projects the global monoclonal antibody market will exceed USD 170 billion by 2025 at a 12.9 percent CAGR….

Using the Right Physicochemical Characterization Tools to Keep the New Variants of SARS-CoV-2 Coronavirus at Bay
| | | | |

Using the Right Physicochemical Characterization Tools to Keep the New Variants of SARS-CoV-2 Coronavirus at Bay

Bylejin.varghese

The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be a global health concern. At the time of writing, the number of diagnosed cases around the world has exceeded 194 million with Asia recording over 60.5 million. At the end of 2020, the emergence of fast-spreading SARS-CoV-2 mutant strains…

Keeping Track of the Latest Innovations in EV Lithium-Ion Battery Manufacturing
| | | | |

Keeping Track of the Latest Innovations in EV Lithium-Ion Battery Manufacturing

Bylejin.varghese

Despite its impressive growth in recent years, the EV lithium-ion battery (LIB) industry still has some catching up to do to reach the levels of maturity of consumer battery manufacturing and the mainstream automotive industry. There are many varieties of materials and electrochemical couples available in the present lithium-ion battery market. Many manufacturers are designing…

Characterization of Microplastics using Morphologically-Directed Raman Spectroscopy
| | |

Characterization of Microplastics using Morphologically-Directed Raman Spectroscopy

Bylejin.varghese

Downloads Characterization of Microplastics using Morphologically-Directed Raman Spectroscopy by Malvern Panalytical (PDF, 2 MB) Microplastics are a global environmental and potential health issue. They can be released directly from the production or use of consumer products or the fragmentation of larger plastic objects. Geographically microplastics are present everywhere on Earth and have pervaded our food…

How PROTACs Can Help Eradicate Cancer With Our Body’s Own Recycling System
| | | | |

How PROTACs Can Help Eradicate Cancer With Our Body’s Own Recycling System

Bylejin.varghese

The ubiquitin proteasome system (UPS) is the body’s built-in garbage recycling system. Its function is to degrade unwanted, used, or misfolded proteins back into amino acids, and scientists have recently found a way to use this to fight against cancer. The newly popularized small molecule proteolysis-targeting chimeras (PROTACs) acts on the UPS by tagging cancerous…

4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments
| | | |

4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments

Bylejin.varghese

During an SPR experiment, there are many experimental parameters that must be controlled in order to optimize the performance of the system and to generate high-quality binding data. Of these parameters, minimizing non-specific binding (NSB) is one of the most imperative as it can directly affect the accuracy of your kinetic data. SPR experiments typically…

Synthesis and Characterization of Antibody Drug Conjugates
| | | | |

Synthesis and Characterization of Antibody Drug Conjugates

Bylejin.varghese

Antibody drug conjugates (ADCs) are attracting a tremendous amount of attention because they have the potential to revolutionize cancer treatment. Chemical ligation of cytotoxic agents to monoclonal antibodies allows the selective delivery of cytotoxins to tumor cells thereby potentially mitigating some of the damaging side-effects of cancer chemotherapy. There are currently nine FDA-approved ADCs and…

Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists
| | | |

Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists

Bylejin.varghese

SPR is a label-free technique that enables you to get in depth information on how two biomolecules are interacting. This is done on a sensor inside of an instrument such as the OpenSPR or Alto. SPR instruments are primarily used to measure binding affinity and kinetics, and are becoming increasingly more affordable and accessible as…