Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis
| | |

Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis

Bylejin.varghese

Quick, label-free methods with minimal sample prep for simultaneously measuring rAAV aggregation, capsid charge, and particle concentration. Date recorded: July 15, 2021 Viral vectors, such as Recombinant Adeno-Associated Viruses (rAAV) Adenoviruses (AV) are increasingly being used in the development of gene therapies and vaccines. The efficient development of viral vectors order to develop effective therapies and…

Master Lipid Nanoparticle & Liposome Characterization with Light Scattering
| | |

Master Lipid Nanoparticle & Liposome Characterization with Light Scattering

Bylejin.varghese

Recent breakthroughs with mRNA-based vaccines highlight the potential of lipid-based particles as powerful and versatile delivery vectors for vaccines and gene therapies. Used to measure particle size, stability, zeta potential and particle concentration, light scattering techniques are used extensively in the characterization of lipid nanoparticle and liposome research. The Zetasizer range of light scattering instruments…

4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments
| | | |

4 Ways To Reduce Non-specific Binding in Surface Plasmon Resonance Experiments

Bylejin.varghese

During an SPR experiment, there are many experimental parameters that must be controlled in order to optimize the performance of the system and to generate high-quality binding data. Of these parameters, minimizing non-specific binding (NSB) is one of the most imperative as it can directly affect the accuracy of your kinetic data. SPR experiments typically…

Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists
| | | |

Surface Plasmon Resonance Sensors: A Comprehensive Guide for Scientists

Bylejin.varghese

SPR is a label-free technique that enables you to get in depth information on how two biomolecules are interacting. This is done on a sensor inside of an instrument such as the OpenSPR or Alto. SPR instruments are primarily used to measure binding affinity and kinetics, and are becoming increasingly more affordable and accessible as…

Analyzing Binding Kinetics: Which Method Is Best for Your Next SPR Experiment?
| | | |

Analyzing Binding Kinetics: Which Method Is Best for Your Next SPR Experiment?

Bylejin.varghese

Read on to learn about the two most common approaches for measuring binding kinetics, multi-cycle kinetics (MCK) and single-cycle kinetics (SCK), and how to select the right one for your interaction.

Insights Into Multi Fluorescence NTA Based EV Characterization – ISEV ‘EV Imaging in Vivo’ Workshop
| | |

Insights Into Multi Fluorescence NTA Based EV Characterization – ISEV ‘EV Imaging in Vivo’ Workshop

Bylejin.varghese

Nanoparticle Tracking Analysis (NTA) with the Particle Metrix ZetaView® instrument family allows fast and reliable measurement of size, concentration, and Zeta Potential of nanoparticles. In contrast to scatter-based particle detection, fluorescence detection capability of the ZetaView® instrument allows the user to get biochemical information about particles. This increases the resolution and discriminatory power of the data. Furthermore,…

The pH Value Is a Key Factor in Cell and Tissue Cultures
| | |

The pH Value Is a Key Factor in Cell and Tissue Cultures

Bylejin.varghese

The term “pH” is derived from either “pondus hydrogenii” or “potentia hydrogenii”. Both are originally Latin phrases, with “pondus hydrogenii” meaning “weight of hydrogen” and “potentia hydrogenii” meaning “power of hydrogen”. The pH value is a non-dimensional number that measures how acidic or alkaline an aqueous solution is. It is defined as the negative decimal…

The Role of SPR in Immunotherapeutic Research
| | |

The Role of SPR in Immunotherapeutic Research

Bylejin.varghese

Understanding the signaling pathways that the biomarkers can activate can help in the development of check-point immunotherapies. Surface Plasmon Resonance (SPR), is a common technique used to identify and elucidate biomarker interactions, signaling pathways, mechanism of interaction, drug discovery and development of therapeutics by providing a label-free, real-time method of determining binding kinetics and binding…

Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS)
| | |

Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS)

Bylejin.varghese

Downloads Biosimilar Comparison and Accelerated Stability Predictions Based on <2% Secondary Structure Differences Using Microfluidic Modulation Spectroscopy (MMS) (PDF, 1 MB) Biosimilar molecules are designed to mimic innovator molecules that have been approved and are available on the market, providing others within the industry an opportunity to create structurally similar therapeutic medicines for greater patient…

Advancing Structural Characterization of Therapeutic Proteins Microfluidic Modulation Spectroscopy with AMGEN
| | | |

Advancing Structural Characterization of Therapeutic Proteins Microfluidic Modulation Spectroscopy with AMGEN

Bylejin.varghese

Existing tools for measuring and monitoring the structure of biotherapeutic molecules during formulation have had until now very significant limitations in sensitivity and have been very difficult to use. In this webinar, Amgen shares data that demonstrates the power of Microfluidic Modulation Spectroscopy (MMS), a novel, fully-automated IR technology, to generate high-quality, reproducible secondary structure…